Cells Series
- Page ID
- 70224
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Erythrocytic (RBC) Series
Rubriblast (Pronormoblast)
- Cell size 14-19 microns
- N/C ratio 4:1
- Round central nucleus
- Red-purple finely stippled, granular chromatin, fine lace-like
- 0-2 nucleoli
- Very basophilic cytoplasm
- No cytoplasmic granules
Rubriblast \(\PageIndex{}\)
Prorubricyte (Basophilic Normoblast)
- Cell size 12 - 15 microns
- N/C ratio 3:1
- Round central nucleus
- Increased granularity of nuclear chromatin (more distinct parachromatin)
- Usually no nucleoli
- Basophilic cytoplasm
- No cytoplasmic granules
Prorubricyte \(\PageIndex{}\)
Rubricyte (Polychromatophilic Normoblast)
- Cell size 10 - 15 microns
- N/C ratio 2:1
- Round central nucleus
- Smaller nucleus with increased condensation of chromatin, very coarse chromatin pattern(distinct parachromatin)
- No nucleoli
- Moderate polychromatophilic cytoplasm -may contain areas of pink hemoglobin near nucleus
- No cytoplasmic granules
Rubricyte \(\PageIndex{}\)
Metarubricyte (Orthochromatic Normoblast)
- Cell size 8 - 12 microns
- N/C ratio 1:1
- Round central nucleus
- Smaller nucleus with pyknotic degeneration, condensed chromatin, no discernible parachromatin (nucleus is solid)
- No nucleoli
- Moderate cytoplasm, lavender - pink due to hemoglobin
- No cytoplasmic granules
Metarubricyte \(\PageIndex{}\)
Reticulocyte (Polychromatophilic Erythrocyte)
- Cell size 7 - 10 microns
- Nucleus absent
- Clear lavender - pink cytoplasm (polychromatophilic).
- Lacks central pallor
- No cytoplasmic granules
Reticulocyte \(\PageIndex{}\)
Retic (Supravital Stain) \(\PageIndex{}\)
Mature Erythrocyte
- Cell size 6-8 microns
- Clear pink cytoplasm
- Central pallor 1/3 of cell diameter
- No cytoplasmic granules
Mature RBC\(\PageIndex{}\)
Myelocytic Series
Myeloblast
- Cell size: 12-20 microns
- N/C ratio 4:1
- Round-oval nucleus, “boxy rounded refrigerator corners”
- Central or eccentric nucleus
- Light red-purple fine meshwork chromatin with no aggregation of material
- 1-5 nucleoli
- Scanty basophilic cytoplasm
- No granules
- May contain Auer rods
Myeloblast \(\PageIndex{}\)
Myeloblast with Auer Rod(s) \(\PageIndex{}\)
Promyelocyte
- Cell size: 15-23 microns
- N/C ratio 3:1
- Round-oval nucleus,
- Central or eccentric nucleus
- Light red-purple fine meshwork chromatin, possibly slight aggregation at nuclear membrane
- 1-3 nucleoli
- Basophilic cytoplasm (may be slightly increased over blast)
- Coarse azurophilic nonspecific (primary) granules
- May contain Auer rods
Promyelocyte \(\PageIndex{}\)
Myelocyte
- Cell size: 10-18 microns
- N/C ratio 2:1 or 1:1
- More round-oval nucleus
- Central or eccentric nucleus
- Red-purple fine chromatin with slightly aggregated pattern
- May or may not have nucleoli
- Moderate bluish - pink cytoplasm
- Nonspecific (primary) and specific (secondary) granules
Myelocyte \(\PageIndex{}\)
Metamyelocyte
- Cell size: 10-18 microns
- N/C ratio 1:1
- Indented (kidney shaped) nucleus
- Central or eccentric nucleus
- Light blue-purple with chromatin clumps easily distinguishable
- No nucleoli
- Moderate bluish - pink cytoplasm (occasionally clear pink)
- Specific (secondary) granules, (few non-specific granules)
Metamyelocyte \(\PageIndex{}\)
Band Neutrophil
- Cell size: 10-16 microns
- N/C ratio 1:1
- Elongated narrow band shape of uniform thickness, singular lobe
- Central or eccentric nucleus
- Deep blue-purple coarsely granular nuclear chromatin (distinct parachromatin)
- No nucleoli
- Abundant pink cytoplasm
- Fine violet-pink specific granules
Band \(\PageIndex{}\)
Segmented Neutrophil (PMN)
- Cell size: 10-16 microns
- N/C ratio 1:1
- 2-5 distinct nuclear lobes (filament connecting lobes)
- Central or eccentric nucleus
- Deep blue-purple coarsely granular nuclear chromatin (distinct parachromatin)
- No nucleoli
- Abundant pink cytoplasm
- Fine violet-pink specific granules
PMN \(\PageIndex{}\)
Monocytic Series
Monoblast
- Cell size: 12-20 microns
- N/C ratio 4:1
- Round-oval, or slightly folded nucleus
- Central or eccentric nucleus
- Pale red-purple, minimal, fine thready chromatin
- Usually 1-2 nucleoli
- Moderate basophilic cytoplasm, regular border
- No granules
- May contain Auer rods
Monoblast \(\PageIndex{}\)
Promonocyte
- Cell size: 10-16 microns
- N/C ratio 3:1 or 2:1
- Round nucleus with chromatin creases or cerebriform folding more distinct
- Central or eccentric nucleus
- Pale red-purple, very fine pattern, (aerated network of threads)
- 0-2 nucleoli
- Paler opaque gray-blue cytoplasm, more abundant, may see pseudopodia and vacuoles
- Contains very fine red dust-like granules
Promonocyte \(\PageIndex{}\)
Monocyte
- Cell size: 12-20 microns
- N/C ratio 2:1 or 1:1
- Cerebriform or horseshoe shaped nucleus
- Central nucleus
- Blue-purple, fine reticular nuclear chromatin (less distinct parachromatin)
- 0-2 Nucleoli
- Abundant pale gray-blue cytoplasm
- May see pseudopodia and vacuoles
- More numerous very fine (dust like) red granules evenly dispersed
Monocyte \(\PageIndex{}\)
Lymphocytic Series
Lymphoblast
- Cell size: 10-20 microns
- N/C ratio 4:1
- Round or oval nucleus
- Central or eccentric nucleus
- Undifferentiated sparse red purple chromatin
- 1-2 indistinct nucleoli
- Scanty, often nearly absent, clear basophilic cytoplasm
- No granules
Lymphoblast \(\PageIndex{}\)
Prolymphocyte
- Cell size: 9 - 18 microns
- N/C ratio 4:1 (occasionally 3:1)
- Round or oval and flat nucleus
- Central or eccentric nucleus
- Combination of condensed clumped blue-purple chromatin with red-purple parachromatin
- 0-1 very distinct nucleoli
- Often scanty, but visible clear basophilic cytoplasm
- No granules
Prolymphocyte \(\PageIndex{}\)
Lymphocyte
- Cell size: 7-18 microns (usually 9-12)
- N/C ratio 4:1 (occasionally 3:1)
- Round or indented nucleus
- Eccentric nucleus often with scanty cytoplasm
- Homogeneous, coarse blue-purple nuclear chromatin (smudged)
- Nucleoli usually absent, rarely one seen in mature forms
- Light to dark blue cytoplasm, scanty to moderate
- Occasionally a few azurophilic granules seen
Lymphocyte \(\PageIndex{}\)
Plasmacytoid Lymphocyte
- Activated B- Lymph
- Generally considered a lymph transitioning to a plasma cell
- Slightly larger with darker royal blue cytoplasm and more heavily clumped chromatin
- May lack very eccentric nucleus and well developed golgi of plasma cell
Plasmacytoid Lymph \(\PageIndex{1}\)
Plasma Cell
- Cell size: 8 - 20 microns
- N/C ratio 2:1 or 1:1
- Round or oval nucleus
- Usually eccentric nucleus
- Blue - purple, dense chromatin with large clumps near nuclear margin
- No nucleoli
- Moderate, basophilic cytoplasm
- perinuclear clear zone adjacent to nucleus
- May contain vacuoles
- No granules
- May see Russell Bodies
Plasma Cell \(\PageIndex{}\)
Thrombocytic (Platelet) Series
Megakaryoblast
- Cell size: 15 - 50 microns
- N/C ratio 4:1
- Usually single round, oval or kidney shaped nucleus (rarely multinucleated)
- Central or eccentric nucleus
- Red- purple, fine chromatin with distinct “smudged” clumps
- 1-5 nucleoli
- Varying shades of blue, amount varies from small to abundant with pseudopodia frequently
- Usually no granules
Promegakaryocyte
- Cell size: 20-80 microns
- N/C ratio 4:1 to 1:1
- Usually 2 - 4 or 8 nuclear lobes
- Central or eccentric nucleus
- Red- purple, increased granularity of nuclear chromatin
- Usually less nucleoli then blast
- Usually abundant cytoplasm with pseudopodia, usually basophilic, but paler as maturity approaches
- Fine azurophilic granules most adjacent to the nucleus
Megakaryocyte
- Cell size: 35 - 160 microns
- N/C ratio 1:2 to 1:3
- Usually 8 nuclear lobes (2 or more, but may have 16 or 32)
- Central or eccentric nucleus
- Blue - purple, granular chromatin
- No nucleoli
- Abundant pale blue (with pink cast)
- May exhibit pseudopodia and shedding platelets
- Numerous fine azurophilic granules, evenly distributed
Megakaryocyte \(\PageIndex{1}\)
Megakaryocyte \(\PageIndex{2}\)
Platelet
- Cell size: 2-4 um
- Light blue, fragment of megakaryocyte cytoplasm
- Evenly dispersed, fine red-purple granules
Platelet \(\PageIndex{}\)